| Date | Home | Away | Result |
|---|---|---|---|
| 12/5/25 1:30 PM | Salford City | Leyton Orient | 4-0 |
| 12/6/25 9:00 AM | Exeter City | Wycombe Wanderers | 4-0 |
| 12/6/25 9:00 AM | Cheltenham Town | Buxton FC | 6-2 |
| 12/6/25 9:00 AM | Accrington Stanley | Mansfield Town | 3-5a.PSO |
| 12/6/25 9:00 AM | Stockport County | Cambridge United | 4-5a.PSO |
| 12/6/25 9:00 AM | Milton Keynes Dons FC | Oldham Athletic | 3-1 |
| 12/6/25 9:00 AM | Wigan Athletic | Barrow FC | 6-5a.PSO |
| 12/6/25 9:00 AM | Fleetwood Town | Luton Town | 6-4a.PSO |
| 12/6/25 9:00 AM | Swindon Town | Bolton Wanderers | 4-0 |
| 12/6/25 9:00 AM | Chelmsford City | Weston-Super-Mare | 0-2 |
| 12/6/25 9:00 AM | Peterborough United | Barnsley FC | 0-1 |
| 12/6/25 9:00 AM | Grimsby Town | Wealdstone FC | 4-0 |
| 12/6/25 9:00 AM | FC Port Vale | Bristol Rovers | 1-0 |
| 12/6/25 11:15 AM | Sutton United | Shrewsbury Town | 1-2a.e.t |
| 12/6/25 1:30 PM | FC Chesterfield | Doncaster Rovers | 1-2 |
| 12/7/25 6:30 AM | Slough Town FC | Macclesfield FC | 1-3a.e.t |
| 12/7/25 8:30 AM | Boreham Wood | Newport County | 3-0 |
| 12/7/25 9:30 AM | FC Gateshead | FC Walsall | 0-2 |
| 12/7/25 11:30 AM | FC Blackpool | Carlisle United | 4-1 |
| 12/8/25 1:30 PM | Brackley Town | Burton Albion | 1-3 |